Journal: Oncology Research

Article Title: STC2+ Malignant Cell State Associated with EMT, Tumor Microenvironment Remodeling, and Poor Prognosis Revealed by Single-Cell and Spatial Transcriptomics in Colorectal Cancer

doi: 10.32604/or.2025.070143

Figure Lengend Snippet: Multifaceted description of STC2 expression and how it relates to molecular and clinical characteristics in CRC. ( A ) STC2 mRNA expression density plots between tumor and normal tissues from four different GEO datasets ( GSE18105 , GSE21510 , GSE39582 , and GSE87211 ). ( B ) Immunofluorescence illustrating the subcellular location of STC2. Following the standard four-color code of HPA, the staining displays the target protein (STC2) in green, microtubules in red, and the nucleus in blue. ( C ) A comparison of the normal colon epithelial cell line NCM460 with a panel of CRC cell lines’ relative expression of STC2. ( D ) The TCGA-CRC cohort’s median expression of STC2 (Z-score) indicates correlation with the progression of the disease across the various clinical stages (Stages I–IV). ( E ) Violin plots illustrating STC2 expression levels among four distinct genomic instability subtypes: chromosomal instability (CIN), genomically stable (GS), hypermutated-indel (HM-indel), and hypermutated-single nucleotide variant (HM-SNV). The p -value indicates a significant difference across the groups. ( F ) Heatmap to illustrate the correlation between STC2 expression and mutation of pivotal CRC drivers (APC, BRAF, and TP53). Samples (columns) are ordered based on decreasing expression of STC2 (top bar), and each gene’s mutation status is marked (1: mutated; 0: wild-type). ( G ) Correlation heatmap indicating the association between STC2 expression quartiles (Q1 representing the highest 25% expression) and various signatures of the immune response and genome state. (*** p < 0.001; **** p < 0.0001)

Article Snippet: Human CRC cell lines SW480 (CL-0223B), Caco2 (CL-0050), HCT116 (CL-0096), LoVo (CL-0144), SW620 (CL-0225B), and normal human colon mucosal epithelial cells (NCM460) (CL-0393) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Immunofluorescence, Staining, Comparison, Variant Assay, Mutagenesis

Journal: Frontiers in Microbiology

Article Title: Identification of differentially expressed HERV-K(HML-2) loci in colorectal cancer

doi: 10.3389/fmicb.2023.1192900

Figure Lengend Snippet: (A–C) Comparing the HERV-K (HML-2) gag , pol , and env gene in colorectal cancer cell lines HCT116, HT29, SW480, and the human intestinal epithelial cell NCM460. Experiments were repeated three times to ensure that the results were replicable. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The human colon mucosal epithelial cell line, NCM460, and three CRC cell lines, HCT116, HT29, and SW480, were obtained from iCell Bioscience Inc. (Shanghai, China) and Procell Life Science Technology Ltd. (Wuhan, China), respectively.

Techniques: